Composite

Part:BBa_K1300001:Design

Designed by: Katty Wu   Group: iGEM14_StuyGem_NYC   (2014-06-16)


pTet promoter + Lock (crRNA) + ccdB + dterm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 655


Design Notes

This sequence is a comprised of four different components: a constitutive promoter (pTet), lock (cis sequence), coding ccdB (without RBS), and a double terminator. The part sequences were placed one right after the other.

The pTet + cis sequence (BBa_J01060) was based on the composition part from the 2005 Berkeley iGEM team. The coding ccdB was based on the minimal ccdB gene (BBa_K414005) from 2010 Nevada iGEM team. We took the minimal gene and removed the RBS. The double terminator (BBa_B0015) was also from the iGEM registry.

Source

Built from existing parts in the registry though BioBrick assembly. 

pTet + lock (cis sequence) [1] & coding ccdB [2] & double terminator [3]

References